The detection and characterization of biomolecular interactions, in particular protein-protein and protein-ligand interactions as well as their formation dynamics and kinetics, are essential tasks in biochemical and pharmacological research.
AUC provides superb capabilities for analyzing and obtaining complex stoichiometries, dissociation constants, and reaction kinetics of relevant systems, such as antibody-antigen, drug-receptor, and therapeutic protein-receptor binding.
Nanolytics offers the most advanced multiplex (multi-signal) analysis resources in AUC, i.e. 4D AUC and AIDA along with the expertise in applying specific data algorithms. This ensures optimal conditions for tackling multi-component systems and characterizing their biomolecular interactions.
CASE STUDY: Investigation of the complex formation between antibodies and antigen in a two-component mixture.
The complex formation of an antibody/antigen pair was analysed in an ab:ag mixture (with a molar excess of the antigen) using multi-signal ck(s) analysis for multi-component mixtures.
The concentration profiles of the two components – antigen (red) and antibody (blue) – are deconvoluted. The putative stoichiometry of homo- and hetero-complexes is obtained according to the molar ab:ag ratio as well the apparent molar mass calculated from the sedimentation coefficients at the respective peak maxima. Here, both an excess of free antigen monomers as well as a mixture of putative 1:2 and 1:3 ab:ag complexes are detected.